Features & Benefits
- K-12 strain of Escherichia coli in a tube
- Gram_ and motile rods
- Cultured in nutrient broth at 37 C
- Enough material for a class of 30 students
- Guaranteed as to species and purity
- Leading supplier of science teaching materials
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Escherichia coli, K-12 Strain, Living, Nutrient Broth, Tube
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This culture is the K-12 strain of Escherichia coli. It is Gram_, motile rods, and cultured in nutrient broth at 37 C in a tube.
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What Stands Out
Live Culture
This product offers a living culture of Escherichia coli K-12 strain, ideal for research applications requiring active bacterial growth and behavior studies.
Nutrient Broth
Cultured in nutrient broth, it supports robust growth and optimal health of the bacteria, ensuring reliable and consistent results for various experiments.
Versatile Use
Designed for laboratory research, this strain is perfect for gene cloning, protein expression, and metabolic studies, providing researchers with a dependable tool for diverse microbiology applications.
Product Details
| Package Weight | 1.27 Pound |
Who Should Buy?
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Educational Institutions
Ideal for biology labs in schools and universities for teaching microbiology and laboratory techniques.
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Research Laboratories
Suitable for genetic, biochemical, and pharmaceutical research that requires a controlled strain of E. coli.
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Biotechnology Companies
Great for companies focused on genetic engineering, synthetic biology, and protein expression using E. coli.
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Food Industry
Not suitable for food safety testing or production due to potential pathogenic strains of E. coli.
Product Description
Escherichia coli, K-12 Strain, Living, Nutrient Broth, Tube
Dietary Supplement Disclaimer
Statements regarding dietary supplements have not been evaluated by the Food and Drug Administration and are not intended to diagnose, treat, cure, or prevent any disease or health condition.
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Customer Questions & Answers
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Question:
What is Escherichia coli, K-12 Strain used for?
Answer: Escherichia coli, K-12 Strain is primarily utilized as a model organism in molecular biology and genetic research. This specific strain is non-pathogenic and serves as a suitable host for cloning and expressing recombinant proteins, allowing researchers to study gene functions and metabolic pathways. Scientists often use it in various applications such as development of antibiotics, vaccines, and in biotechnological innovations. -
Question:
Is the K-12 strain of E. coli safe for laboratory use?
Answer: Yes, the K-12 strain of E. coli is considered safe for laboratory use as it is a non-pathogenic variant. It has been extensively studied and is one of the most utilized strains in research settings. Due to its non-virulent nature, it poses minimal risks to lab personnel and the environment, making it ideal for educational purposes and laboratory experiments. -
Question:
What are the characteristics of the K-12 strain of E. coli?
Answer: The K-12 strain of E. coli is characterized by its ability to utilize various carbon sources, rapid growth rates, and genetic tractability. These traits make it an excellent candidate for host organisms in genetic engineering and synthetic biology. Its well-characterized genome allows for extensive molecular manipulation, providing researchers with valuable insights into bacterial physiology and genetics. -
Question:
How is the K-12 strain maintained in the lab?
Answer: The K-12 strain can be maintained in a laboratory setting by regularly culturing it in nutrient broth or agar plates under appropriate conditions. It's common to store the strain at low temperatures or freeze it in glycerol stocks to preserve its viability over time. Regular sub-culturing enables researchers to keep the strain active and ready for various experiments. -
Question:
What applications does Escherichia coli K-12 have in biotechnology?
Answer: Escherichia coli K-12 has a vast array of applications in biotechnology, including the production of insulin, growth hormones, and enzymes. Researchers harness its capabilities to perform gene cloning, protein expression, and metabolic engineering. The strain's versatility makes it a cornerstone organism in the field, providing essential insights that drive advancements in pharmaceuticals and synthetic biology. -
Question:
Can K-12 E. coli be genetically modified?
Answer: Yes, K-12 E. coli is frequently genetically modified to enhance its capabilities for specific research needs. By using various transformation techniques, scientists can introduce foreign DNA into the bacteria to study gene function or to produce desired proteins. This adaptability makes the K-12 strain a powerful tool for molecular biology and genetic research. -
Question:
What kind of nutrient broth is suitable for culturing K-12 strain?
Answer: For culturing K-12 strain E. coli, a nutrient broth rich in amino acids, vitamins, and sugars is suitable. Commonly used broths include Luria-Bertani (LB) broth, which supports rapid growth and maintenance of E. coli cultures. This broth ensures optimal growth conditions, allowing for efficient cell division and protein expression in laboratory experiments. -
Question:
What precautions should be taken when handling live K-12 strain?
Answer: When handling live K-12 E. coli, standard laboratory safety precautions should be followed. This includes wearing appropriate personal protective equipment (PPE), utilizing biosafety cabinets, and adhering to sterile techniques to prevent contamination. While K-12 is a non-pathogenic strain, practicing good laboratory hygiene ensures a safe environment and prevents accidental cross-contamination with other microbes. -
Question:
What is the shelf life of K-12 E. coli in nutrient broth?
Answer: The shelf life of K-12 E. coli in nutrient broth generally depends on storage conditions. When properly refrigerated, the culture can remain viable for several weeks. However, to maintain long-term stability, it's advisable to store the strain in glycerol stocks at ultra-low temperatures, where it can remain viable for years, allowing researchers to revive it for future experiments. -
Question:
Where can I buy Escherichia coli, K-12 Strain, Living, Nutrient Broth, TubeF?
Answer: You can purchase Escherichia coli, K-12 Strain, Living, Nutrient Broth, TubeF from Ubuy in Sri Lanka. Ubuy offers a variety of laboratory products, including biological strains and nutrient broths, ensuring you have access to quality materials for your research needs.
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